Dynamics of the nuclear lamina during differentiation and EMT

Project participants

Julia Madsen-Østerbye, Aurélie Bellanger, Nolwenn Briand, Mohamed Abdelhalim, Sarah Hazel Pickering, Kristin Vekterud

 

Image: the nuclear envelope with bound heterochromatin, and 3D chromatin modeling at the nuclear periphery - TEM and Chrom3D modeling images

Ongoing research

  • Regulation of nuclear envelope interactions with chromatin through LADs and sub-LAD microdomains during adipose differentiation
  • Role of the nuclear lamina on EMT
  • Impact of lamin A mutations causing lipodystrophic laminopathies on nuclear integrity, lamina-chromatin interactions and adipose stem cell fate

Our work relies on mechanistic cell biology studies, including fixed- and live-cell microscopy, genomics, functional and proteomics approaches using engineered cell lines and primary adipose stem cells.

Recent findings

  • High-order rearrangement of chromatin domains when disrupting lamin-chromatin contacts
  • Differential gene expression in constitutive LADs
  • variable LADs and cellular identity