- Project leader:Aurélie Bellanger (lamin A/C and EMT), Marie Rogne (microProteins, TF targeting)
- Info:3D genome organization is altered in cancers. Levels of lamin A/C often vary between and within tumors, but their implications in cancer development are unclear. We are studying the role of A-type lamins, as genome organizers, in breast cancer progression. In addition, pioneer transcription factors, which can initiate new gene expression programs, are commonly dysregulated in cancer, and are often linked to cancer aggressiveness. We are exploiting properties of microProteins and how they may affect transcription factors to understand and target transcription factor activity in aggressive bone metastases.
Project participants
Aurélie Bellanger, Marie Rogne, Emily Martinsen, Mohamed Abdelhalim, Tasmia Jinnurine; collaborations with Jonas Paulsen, University of Oslo; Pioneer Research AS, Oslo; Erwan Delbarre (lab alumnus), Oslo Metropolitan University; David Tremethick, Australian National University, Canberra
Ongoing research
- Nuclear lamin A/C and transcription factor dynamics in a breast cancer model of EMT
- Relationship between the nuclear lamina, 3D genome organization and breast cancer progression to metastasis
- Inhibition of transcription factors by microProteins in metastatic cancers
- Histone variant H3.3K27M mutation and chromatin organization in pediatric glioblastomas (DIPGs)
- Regulation of gene expression at the nuclear lamina
Recent findings
- Re-wiring of transcription factor networks by lamin A/C in a breast cancer EMT model (Bellanger, submitted)
- Remodeling of chromatin at the nuclear periphery by the histone H3.3K27M mutation in DIPG-derived cells (in preparation)
- A polymer model of chromatin to infer chromatin behavior at the nuclear lamina (Brunet 2021 Nucleus)
- Chrom3D: a platform for 3D genome modeling from Hi-C and LAD data (Paulsen 2017 Genome Biol; Paulsen 2018 Nature Protoc)
- Chrom3D on github: https://github.com/Chrom3D/Chrom3D
- Mechanisms of H3.3 deposition into chromatin (Delbarre 2013 Genome Res; Ivanauskiene 2014 Genome Res); (Delbarre 2017 Genome Res)