3D genome modeling

3D genome modeling

Project leaders: Philippe Collas and Jonas Paulsen

 

3D genome organization of the genom

e influences cell- and time-specific blueprints of gene expression. Some aspects of 3D genome conformation vary between cell types, su

ggesting developmental regulation – that is, regulation in a 4D space where the 4th component is time. 3D genome conformation entails interactions between chromosomes, forming interactions ‘hubs’ called topologically-associating domains (TADs). At the nuclear periphery, chromosomes interact with the nuclear lamina through lamin-associated domains (LADs). These interactions are dynamic during cell differentiation. For a recent review from our lab, see Sekelja et al. 2016 Genome Biol.
We are investigating links between cellular metabolism and changes in spatial genome conformation during differentiation of adipose stem cells in normal and pathological conditions.

Ongoing research:

  • Computational methods for 3D and 4D modeling of genome architecture
  • Functional relationships between 3D chromatin folding, nuclear envelope-chromatin interactions, epigenetic states and lineage-specific differentiation capacity

Recent achievements: